PDT in oral hygiene- a split mouth randomized trial
Nikinmaa S, Meurman J, Moilanen N, Sorsa T, Rantala J, Alapulli H, Kankuri E, Kotiranta A, Auvinen P, Pätilä T.
The effects of indocyanine green-mediated photodynamic therapy on the development of plaque, plaque bacteriological ecology, and early periodontitis markers - A randomised study
Antimicrobial photodynamic therapy (aPDT) has been introduced as an adjunct method for dental hygiene. Although antibacterial and antiplaque effects resulting from aPDT
Fifteen healthy adults were assigned to the study. Upper premolars (4. and 5.) were examined on both sides of the maxilla. After meticulous scaling and root planing, the maxillary dental arch was left without any mechanical cleaning for four days. Randomisation of the treatment side of the upper dental arch was performed, and following the initial sample collection, the mouth was rinsed with indocyanine green (ICG), and 100J/cm2 of 810 nm light was subsequently applied for eight minutes. The treatment was repeated daily for four days. ICG localisation after the mou
Antimicrobial photodynamic therapy resulted in a significant reduction of plaque formation. An analysis of the 16S rRNA sequencing found a reduction in the Streptococcus, Acinetobacerial, Capnocytophagal, and Rothia bacteria species and a gain in Neisseria and Hemophilus ba
In conclusion, ICG -based aPDT is effective and reduces the amount of known oral pathogens, with compensated bacterial growth in species associated with good oral health, but without a change in overall bacterial diversity. The treatment can be applied specifically to dental plaque, and the anti-inflammatory effect may prevent the development of early gingivitis.
Dual light provides a sustained antibacterial effect
Nikinmaa S, Meurman J, Sorsa T, Rantala J, Pätilä T
Daily administered dual light photodynamic therapy provides a sustained antibacterial effect in biofilm and prohibits Streptococcus mutans adaptation
Materials and Methods
Biofilm was grown on the bottom of well plates by adding diluted Streptococcus mutans
On a single-day biofilm, dual-light was significantly more efficient than aBL or aPDT, although all modalities were bactericidal. On a four-day maturated biofilm, single exposure of aPDT or dual-light aPDT was more efficient than aBL, resulting in a four logarithmic scale reduction in CFUs. Surprisingly, when the same amount of aPDT was given repeatedly, on a daily basis, on the four-day biofilm, a significantly improved bacterial viability was observed. Similar, but milder response was seen after repetitive aBL application.
When aPDT is administered repeatedly to S. mutans-biofilm, a single wavelength-based aBL or aPDT